MEC BIO2006-26132-E, 2006-2007

Principal Investigator: José Luis Micol.
Investigators: María Rosa Ponce.
Instituto de Bioingeniería, Universidad Miguel Hernández.

Gene mapping facility

We already implemented a mapping facility for untagged mutations in Arabidopsis thaliana. Our service has been free of charge for the community of Spanish researchers in this plant species and has been funded for the last three years (2003-2006) by the Ministry of Education and Science. We ask for funding to keep this facility at work for the next two years. In a first step of the process (of two weeks), we will extract the DNA of at least 50 F2 individuals from an outcross involving a homozygous plant for a mutation of interest. The DNA will be used to test linkage to 32 SSLP markers, which will allow a low-resolution mapping yielding a rough map position within a 15 cM interval. In a second step (2-3 months), DNA from 450 additional F2 individuals will be extracted to make possible a high resolution mapping defining a candidate interval of about 100 kb.